5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. BMG Labtech - THERMOstar. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. The most published microplate reader control and data analysis software. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Important things to consider when purchasing a multimode microplate reader. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Videos & Webinars. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. 0A-Sunnyvale, CA-Made in USAMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Details. #4TI-0655) Microplates. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Microplate Reader, Multimode Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. . ” So, acquisition and analysis come from one software system. StakMax Handling Stacker Base Unit. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. The EMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. System setup and calibration are controlled from within SoftMax Pro Software. Other features and benefits include: Eliminate changing out filters. Supplier: Molecular Devices. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 0. 1 - 16 of 69. FlexIII. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. The temperature in the plate chamber is ambient and isothermal. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. 96-well plate and 12,500-20,000. Check software compatibility with Windows 10 and locate your software installer. 2. For biochemical assays, the washer removes unbound material and unreacted reagents. and it is unrealistic to expect one experiment to occupy a plate reader for multiple hours or days. Wait 20 seconds. ELISA Plate Readers / Microplate Readers. Shop now. 0. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. . 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1. The former have been the traditional domain of plate readers and great strides have been. Also refer to the Multimode Reader Comparison Chart Features. Cell viability는 세포막 Integrity 또는. The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. W×D×H. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Read plate standard and mix. 3860 N First Street San Jose,CA 95134 USA +1 408. Avoid losing high signal. 0 . SpectraMax® Microplate Readers and SoftMax® Pro Software. This multi plate reader is excellent to work with for checking the U. Recombinant protein expression Nucleocapsid. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). 3860 N First Street San Jose, CA 95134. Company Info. The Molecular Devices FilterMax F5 (replacing our old Analyst plate reader) is a versatile instrument that offers detection based on fluorescence, fluorescence polarization, luminescence, absorbance, and time resolved FRET. Every step is optimized to simplify analysis and reporting to support our microplate readers. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. MycoAlert Substrate was then added to the wells, and the plate was incubated for 10 minutes at room temperature. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. 5 sec. DOCUMENTATION. 100 μL of blank or endotoxin standard (in. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. Plate. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Evaluation of BioTek's Synergy H4 Plate Reader for Fluorescent Detection. com 4. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Catalog Number: (89212-396) Supplier: Molecular Devices. (7) Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Used. 1-10 sec. Lastly, we demonstrate the utility of this novel fluorescence-based assay to evaluate the activities of. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Unplug the power cable from the back of the instrument. Incubate at RT. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Luminescence is the emission of light by a substance as a result of a chemical reaction (chemiluminescence) or an enzymatic reaction (bioluminescence). The assay is easy to set up and the range of potential analytes is vast, but the assay procedure is time consuming and labor intensive. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with allSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Figure 1. The Back Panel Figure 1. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. The guide also covers the software features, the specifications, and the accessories of the systems. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 0200-6263. 1. FLIPR Calcium 4 Assay Kit. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. The plate reader optics are tunable for absorbance,. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Instrument settings can be saved as a protocol file. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Imaging with the MiniMax 300 Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. Plate Reader-2 features a wide range of high-performance multi-mode reader capabilities ideal for life science research on the International Space Station (ISS. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Absorbance, fluorescence, and luminescence detection with upgradeable high-performance capabilities. 40-plate magazine (pair) 0200-6017. A control well was set up to verify the accuracy of the standard curve. Molecular Devices' microplate readers. Upgradeable modules are also available. . Open the top cover of the instrument. 5. Select the Driver tab. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. These systems are hybrids that allow you to choose between. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. M2. Company Molecular Devices, LLC. . Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. 340 PC384. Choose the desired plate type, using the upper dropdownThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 2. Integrate . MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPrimary considerations when choosing a microplate reader are throughput and flexibility. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 9. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. A. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Turn the reader on and secure the USB cable. Fixed cells stained with fluoresceinconjugated anti-VCAM are identified with the Marker Expression Protocol. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableSpectraMax ABS Plus Microplate Reader (Molecular Devices cat. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Cells in each. 1. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. 747. 0 . Plate Reader. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The adapter is required for optimum performance with standard 96-well and 384-. It may be named "Pothos". Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. Molecular Devices, LLC. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. 2. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. Extended linear dynamic range. Scanning options are endpoint, kinetic, spectral. Learn how you can utilize microplate readers to drive landmark discoveries. Jun 01, 2004. 5032018C 8 Chapter1:Introduction TheEMax® PlusMicroplateReaderisafilter-basedabsorbancemicroplatereaderwitheight standardfiltersforawiderangeofapplications. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. #0200-7011) Instrument setup SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. When the user applies a set temperature of 37°C, the top is heated to 37. 0) OD (500 to 1100) RFU (5. 1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Plate Formats 6 to 1536 wells. #6590-0118) Filter 665/10 (Molecular Devices cat. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Find our technical support information along with contact details. Step 4: Incubate at room temperature for 1 hour, protected from light. Flexible integration options. Restart the system. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 9. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 0 × 103 to 2. Ratios for each sample were calculated as Read B/Read A. 0200-6262. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. +1 800-635-5577. Turn on Plate Reader and wait for it to complete its start-up routine. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. 4: Test tube cover. Retrieving Each. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Step 1: Open the SoftMax Pro IMAP FP protocol. 0%, raw data units = counts/sec. The system provides automation for up to 50 microplates for easy walkaway automation. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Increase productivity with patented microplate reader technologies. 1. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Our microplate readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. 303 unable to cal dark current. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 020 OD 2. Uncontrolled Copy for Training and Reference Purposes Only . Detection cartridge modularity allows for easy addition of new read modes within minutes without. Shop a wide selection of Molecular Devices FLIPR TETRA System's on LabX. 0077 OD 0. Cells were thawed and propagated according to the recommended method specified by the supplier. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Research-grade results. Molecular Devices’ SpectraDrop TM MicroVolume Microplate for SpectraMax ® microplate readers offers higher throughput than any other low-volume microplate, with up to 64 samples per plate. PURPOSE. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Search. 9. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The direct transfer capability reduces We would like to show you a description here but the site won’t allow us. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Pre-defined protocols. Learn how Molecular Devices FlexStation 3 Multi-Mode Microplate Reader provides life science industry solutions. Description: ScanLater* Western Blot Detection Cartridge, enables the first western blot detection capability to a microplate reader, Time-resolved fluorescence detection, Xenon flash lamp excitation source. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 5 µL of each sample was pipetted into a 96-well. 1 sec. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Changing Plate Hold-Down Devices 123 Cell Reservoir 125 Chapter 7: Calibration and Signal Test 127. D 1. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. 9000-0756. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. Ensure data accuracy of your reader. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Read height should be optimized for Vmax® plate reader specifications. With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax® Mini reader . FlexStation™ II Systems provide integrated fluid transfer capability from a source plate or reservoir to an assay plate with concurrent kinetic fluorometric analysis of reactions. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. QuickSync. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Multi-mode Plate Reader. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. The drawing shows a standard microplate of 15 mm height covered with a lid. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The plate is set up for reading and images are acquired according to specified parameters. 4 °C above ambient to 45 °C. Instrument settings can be saved as a protocol file. The plate is set up for reading and images are acquired accordingSpectraMax Readers have the most complete level of product validation and compliance. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. Multimode Microplate Readers. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Expanded wavelength coverageOther readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. 20-plate magazine (pair) 0200-6016. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersMolecular Devices technical support. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Turn the reader off by pulling the power cord. M2. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may beSoftMax Pro GxP Software. July 17, 2023. Learn how you can utilize microplate readers to drive landmark discoveries. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Description Figure 1. the standard plate reader applications, protecting your initial investment. An industry-renowned instrument for monitoring GPCRs and ion channels. Supersedes 1. to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and. Increase productivity with patented microplate reader technologies. Supersedes 1. Plate Formats: 6 to 384-well microplates. 1. Microplate Reader Shelf. The sample is typically placed in a cuvette through which light is sent horizontally. Your applications, your modes, your choice. Find regional support. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. After reading the ELISA plate on both readers, each standard curve was nearly identical. Use all black plate. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. Molecular Devices, LLC. Search. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. This offer includes SpectraMax i3x, SpectraMax. Fluorescence Microplate Reader Comparison Chart. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofThe plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and mixed. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. Molecular Devices Software. AXON Patch-Clamp. Reader Documentation Version No. Turn on Plate Reader and wait for it to complete its start-up routine. Molecular Devices Flourmeteric Imaging. Optimize. This document is provided to customers who have purchased Molecular Devices, Inc. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. Spectranet customer portal. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Versa Max. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. With the discontinuous kinetics feature in SoftMax Pro Software. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. ISM Equipment Classification (Group 1, Class A) This equipment is. Figure 10: The Plate section Template Editor, showing the series set up. D. gene expression with a user-friendly reader. This document is copyright protected and any reproduction of this document, in whole or. If the computer is connected to the Internet: Select Search Automatically for. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human. Flexibility to read a variety of plate formats. To purchase a newer software version,. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 0A Sunnyvale, CA Made in USA Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Gregory et al. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. visible absorbance, fluorescence. We would like to show you a description here but the site won’t allow us. Shortside barcode reader: 0310-5102. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. A single software solution addresses your needs, from plate set-up to image analysis. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. 8×15×8. PathCheck sensor corrects optical pathlength & pipetting variation. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. We would like to show you a description here but the site won’t allow us. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. These robust readers have been placed in labs from. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. 32 was diluted 10-, 100-,. On the back, in the right hand top corner, there. Superior optics Microplate Reader Shelf. 0A Sunnyvale, CA Made in USAFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Download PDF versions of the SpectraMax Microplate Reader User Guides. Latest Citations: For a complete list, please click here. Molecular Devices' microplate readers. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 4: Test tube cover. 400×320×350 mm. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Learn how Molecular Device's VersaMax Microplate Reader can be combined with the Pyrochrome Assay from Associates of Cape Cod, Inc. S. Double-click the unknown device with the yellow warning icon. 1. 0 OD for earlier models. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. For a higher . The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 2. First, as mentioned before, the walkaway time significantly. EMax. 1. 5. coli stock with an OD 600 value of 0. .